Bio 112L                        General Biology I Laboratory                            2007
INSTRUCTORS:

R. PINETTE                          OFFICE: FOLSOM 307  X9481   MWF, 11-12
 J. JOHNSTON                                       SOUTH HALL    X      
LAB MANUAL: PINETTE, R. 2006. A LABORATORY MANUAL FOR
                                 GENERAL BIOLOGY 112. 8th ed.  Updated for 2007.

 I. COURSE OUTLINE     Lab meets once weekly Wednesday 2-5:00 (Pinette)

                                                                                  Thursday 2-5:00 (Johnston)

W/T

TOPIC

TESTS AND ASSIGNMENTS

Aug 29-30

Introduction and Lab Safety & Etiquette

 

Sep 5-6

 1. The Compound Microscope

Quiz#1on Safety/Etiquette

 

Sep 12-13

2. Making Measurements and      

   the  Dissecting Microscope

Quiz#2 on Ex 1/Do metric problems before coming to lab and read instructions for making drawings.

Drawing an Amoeba cell.

Sep 19-20

3. Plant and Animal Cell

    Structure

Drawings of plant and animal cells

Sep 26-27

4. Osmosis 

Quiz#3 on Ex 2 & 3/

Elodea Cell Drawings  

Report I

Oct  3-4

5. Mitosis

Drawings of mitosis figures.

 

Columbus Break (October 6-14)

 

Oct 17-18

6. Mendelian Genetics

Quiz#4 on Ex 4& 5 /Bring your calculator!  (Report 1 Due)

Oct 24-25

7. DNA Extraction/ Spooling
10. Human Karyotyping

Human karyotype

Oct 31/

Nov1

8. Enzyme Activity & Temperature

Quiz#5 on Exercises 6, 7 & 10  

Nov  7-8

9. Alcohol Fermentation

11. Chromatography

Report 2

Nov 14-15

 11. Plant Structure

Leaf, stem & root drawings

 

 

Thanksgiving Break (Nov 21-25)

 

 

Nov 28-29

12. Microevolution

 

Bring your calculator! 

 (Report 2 due)

Dec 5-6

13. Animal Dissection

 

Frog Anatomy Drawings Quiz#6 on Ex 9, 12 & 11

II. Student Evaluation. Your lab and lecture scores will be combined into one    grade for the entire course. The following items constitute 25% of the course grade. See your lecture syllabus for more information on grades.

1 lab report                                                                                   25
Best of 5 drawings and other laboratory work products               25
Best 5 of 6 quizzes at 10 points each                                          50
possible points for the lab                                                           100

III. Laboratory Drawings. A list of materials and guidelines for preparing drawings is given in the introduction to your lab manual.  Each drawing set (except the Amoeba) and the human karyotype is worth five points.   Of the six possible assignments, the lowest score will be dropped.  Drawings for missed labs will not be accepted.

IV.  Quizzes.    Every other week a quiz will be administered over the laboratory
exercises of the previous two weeks.   Each quiz is worth ten points.  The lowest
score of six quizzes will be dropped.  A missed quiz will be counted as your
 lowest score.  There will be no make up quizzes.

V.  Lab Report.  (25 points).  Two lab exercises will be the subject for a lab report during the term.  Half the students will write a report on osmosis from Exercise 4; the other half will write a report on fermentation from Exercise 9.   More information will be provided in the form of a handout at a later date.

VI. General Information

·        Attendance of all laboratory sessions is required.

·        Any student missing more than three laboratory periods will be given an F unless extenuating circumstances can be shown.

·        Missed labs and quizzes can not be made up. 

·        Contact your instructor immediately if you know in advance that you will
miss a laboratory period. Do not wait until the next day or later in the week.

·        Lab drawings are due at the end of the laboratory period.

General Laboratory Safety Guidelines

I. Emergency Equipment and Telephone

Emergency Number:   8-9-1-1

 

Be prepared to give your name, address (181 Main Street, Presque Isle), Folsom Hall, Room 304 and emergency reason.   The dispatcher is able send the appropriate response unit as needed.

 

II. Location of Safety Equipment in Laboratory

 

Shower & Eyewash: 

Rear of lab by windows.

Fire extinguisher:

On the wall on the right side of the dry board.

First aid kit:

On wall near refrigerator.

Fire blanket:

On the wall on the right side of the dry board.

  

III. General Lab Safety Guidelines

 

1.      Wear appropriate clothing and shoes in the laboratory.  If you own a lab coat, bring it to lab.

2.       Avoid loose fitting items of clothing.  Coats and jackets should be placed on bench designated for this purpose.             

3.      Food and drinks are not allowed in the laboratory. 

4.      Keep your workspace free of all unnecessary materials.   Backpacks should be place on the floor near your feet, but not in the aisle.

5.      Shoulder length hair must be tied back in a pony tail to prevent injury from Bunsen burners and to avoid contamination of laboratory materials.

6.      Report spills and accidents immediately to your instructor.

7.      Be sure to follow the instructor’s directions in disposal of all chemicals.

8.      Only aqueous solutions of inorganic chemicals can be poured into sinks.  Organic wastes must be disposed of in appropriate containers marked for this purpose. 

9.      Do not return unused chemicals to the stock bottles.

10.  Dispose of broken glass in yellow receptacle marked for this purpose.

11.  Dispose of razor blades and sharp metals objects in red biohazard receptacle.

12.  Wash your hands with disinfectant soap provided at the sink before leaving lab. 

 

IV. Emergency Procedures for a Laboratory Fire

 

·        Do not use a fire extinguisher unless you are trained to do so.

·        In case of a small fire at your desk, smother with a book or piece of cloth.  Do not use your hands.

·        If your clothes are on fire such as a sleeve, run water over it.  If the fire has spread beyond a small portion of clothing or appears that it will, use the fire blanket.  It is likely that a student will need help with the fire blanket, be prepared to help someone wrap into the fire blanket.  If all else fails drop and roll.

·        If a large fire occurs such as in a waste basket, smother the fire with a jacket or other piece of cloth.

·        If the fire is larger, DO NOT ATTEMPT TO PUT OUT THE FIRE.   Leave the laboratory.  Follow the evacuation plan.  If the alarm has not yet sounded, pull the fire alarm on the left side of hall before fire doors leading to the north stairway and continue to the gathering area designated in the evacuation plan. 

 

V. Electrical Equipment

 

·        Do not spray or splash water in the vicinity of plugged in electrical equipment.

·        If equipment crackles, snaps or begins to smoke, do not attempt to disconnect it, call the instructor immediately.

·        In case of any frayed or broken electrical cords, notify the instructor.

 

VI. First Aid for Minor Injuries.

 

·        Band aids and some basic first aid materials are available in the lab.

·        For spills in the eyes, use the eyewash station.

·        For burns, chill the affected part with cold water and ice as soon as possible.

 

VII. Evacuation Plan

 

Should conditions in the laboratory require evacuation due to an accident, hazardous chemical pill, fire or due to fire alarm sounding elsewhere in the building, students will immediately leave the building by the north stairway. Turn left upon exiting the building and proceed to the lawn across from the parking space on the west side of the Folsom Hall.  It is important that the class gather together at this location for the instructor to confirm that all students in the course have evacuated the building.