Bio 113L                              General Biology II Laboratory                                  2008

 

Instructors:  R. Pinette                                Office:   Folsom 307   MWF 11-12  768-9481

                    J. Johnston                           Office:   South Hall 315                   768-9652

Lab Manual.   Pinette, R. 2004.  A Laboratory Study of Biological Diversity for Biology—Revised for 2008 113. Presque Isle, 2nd Edition. 75 pp.                                       

 

Photo Atlas:  van De Graaff and J Crawley.  2005.  A Photographic Atlas for the Biology

                             Laboratory. 5th ed. Morton Pub. Co. Englewood.  264 pp.

 

Students will need about 50 sheets of 8 1/2 x 11 photocopy paper,  # 2.5 lead

 pencils, colored pencils (green, blue and red) and a ruler. 

 

Course Outline Lab meets once weekly on Thursday:     Pinette (9:25-12:25)   Johnston (1:40-4:40)

DATE

TOPIC

QUIZZES

 

 

 

17-Jan

COURSE INTRODUCTION

 

 

 

 

 24-Jan

1. PROKARYOTES

 

 

 

 

31-Jan

2. PROTOZOA

 

 

 

 

7-Feb

3. ALGAE

QUIZ #1 on Exercises 1 and 2

 

 

 

14-Feb

4. FUNGI

 

 

 

 

 

WINTER BREAK Feb 16 to Feb 26

 

 

 

 

 28-Feb

5. LICHENS AND BRYOPHYTES

QUIZ #2 on Exercises 3 and 4

 

 

 

  6-Mar

6. FERNS

 

 

 

 

13-Mar

7. GYMNOSPERMS

QUIZ #3 on Exercises 5 and 6

 

 

 

 20-Mar

8. ANGIOSPERMS

 

 

 

 

 27-Mar

9. SPONGES AND CNIDARIANS

QUIZ #4 on Exercises 7 and 8

 

 

 

 

SPRING BREAK  March 29--April 6

 

 

 

 

 10-Apl

10. FLATWORMS AND ROUNDWORMS

 

 

 

 

17-Apl

11. MOLLUSKS AND ANNELIDS

QUIZ #5 on Exercises 9 and 10

 

 

 

24-Ap1

12. ARTHROPODS

 

 

 

 

1-May

13. ECHINODERMS AND CHORDATES

QUIZ #6 on Exercises 11 and 12

 

 

 

 

     

 

 

        Student Evaluation

6

 Quizzes at 12 points each (lowest score dropped)

60

13

 Sets worth 3-5 points each (lowest or missed sets dropped)

40

 

                                                      Total possible for lab

      100

 

 

 

      

See course policies attached to lecture syllabus for further information on grades.

 

Lab Quizzes. 

·      There are six 12 point quizzes scheduled every other week on material from the previous two laboratories. These written tests will be given at the beginning of the laboratory period.  The quizzes are largely formulated, verbatim, from post laboratory questions or modified versions of them.  

·      Each quiz will also have 2 points based on recognition of Domain, Kingdom and Phylum/Division. 

·      The lowest quiz score will be dropped.  If you miss a quiz, then that will be your lowest score.   There will be no make up quizzes. 

 

Laboratory Drawings.  Students will be required to make detailed drawings of materials examined during the term. 

·      The guidelines for making the drawings are specified at the beginning of the lab manual

·      Each page of drawings will be graded as these will be grade as 0. + or ++.  ( point values are 0 = 0, +, 0.5; ++ = 1)

·      Drawing sets will be turned at the end of each laboratory period. (Drawings will be accepted for only those labs which you attended.)

·      Your grade will be based on forty drawings.  Additional drawings will be counted as bonus points—up to 10 maximum.

 

Post Laboratory Questions.  Each laboratory exercise is followed by a set of questions intended to help you study for the material.  If you finish the laboratory observations and drawings early, then it is expected that you will work on the post lab questions.   Some of the questions are specific to demonstration materials so it is to your advantage to answer the questions during the laboratory period.   Your responses will not be turned in or graded, so it will benefit you to get feedback on those answers for which you are uncertain.

 

Attendance.  Attendance of each laboratory session is mandatory.   There will be no make-up laboratories. Students who miss more than three labs automatically fail the course unless there are extenuating circumstances.   This must be clearly 

 

 

Divorce.  Too often students divorce lab from lecture.  The laboratory is part and parcel of one course.   It is intended to demonstrate the structures and biological diversity that is discussed in lecture.  When you answer post laboratory questions, prepare drawings and study for lab quizzes, remind yourself of what was discussed in lecture. Conversely, when you study lecture material, remember at what you studied in lab too. 

 

General Laboratory Safety Guidelines Folsom 304

 

I. Emergency Equipment and Telephone

Emergency Number:   8-9-1-1

 

Be prepared to give your name, address (181 Main Street, Presque Isle), building name, room number and nature of emergency.   The dispatcher is able to send an ambulance, police or fire.

 

II. Location of Safety Equipment in Laboratory

 

Eyewash and shower:  In the back of the lab near the windows.

 

Fire extinguishers:        On the wall on the right side of the dry board.

 

First aid kit:                   On the wall near the refrigerator.

 

Fire blanket:                  On the wall on the right side of the dry board.

 

III. General Lab Safety Guidelines

 

1.      Wear appropriate clothing and shoes in the laboratory.  If you own a lab coat, bring it to lab.

2.       Avoid loose fitting items of clothing.  Coats and jackets should be placed on bench designated for this purpose.             

3.      Food and drinks are not allowed in the laboratory. 

4.      Keep your workspace free of all unnecessary materials.   Backpacks should be place on the floor near your feet, but not in the aisle.

5.      Shoulder length hair must be tied back in a pony tail to prevent injury from Bunsen burners and to avoid contamination of laboratory materials.

6.      Report spills and accidents immediately to your instructor.

7.      Be sure to follow the instructor’s directions in disposal of all chemicals.

8.      Only aqueous solutions of inorganic chemicals can be poured into sinks.  Organic wastes must be disposed of in appropriate containers marked for this purpose. 

9.      Do not return unused chemicals to the stock bottles.

10. Dispose of broken glass in yellow receptacle marked for this purpose.

11. Dispose of razor blades and sharp metals objects in red biohazard receptacle.

12. Wash your hands with disinfectant soap provided at the sink before leaving lab. 

 

IV. Emergency Procedures for a Laboratory Fire

 

·        Do not use a fire extinguisher unless you are trained to do so.

·        In case of a small fire at your desk, smother with a book or piece of cloth.  Do not use your hands.

·        If your clothes are on fire, such as a sleeve, run water over it.  If the fire has spread beyond a small portion of clothing or appears that it will, use the fire blanket.  It is likely that a student will need help with the fire blanket, be prepared to help someone wrap into the fire blanket.  If all else fails drop and roll.

·        If a large fire occurs such as in a waste basket, smother the fire with a jacket or other piece of cloth.

·        If the fire is larger, DO NOT ATTEMPT TO PUT OUT THE FIRE.   Leave the laboratory.    Follow the evacuation plan.  If the alarm has not yet sounded, pull the fire alarm on the left side of hall before fire doors leading to the north stairway and continue to the gathering area designated in the evacuation plan. 

 

V. Electrical Equipment

 

·        Do not spray or splash water in the vicinity of electrical equipment which is plugged in.

·        If equipment crackles, snaps or begins to smoke, do not attempt to disconnect it, call the instructor immediately.

·        In case of any frayed or broken electrical cords, notify the instructor.

 

VI. First Aid for Minor Injuries.

 

·        Band aids and some basic first aid materials are available in the lab.

·        For spills in the eyes, use the eyewash stations.

·        For burns, chill the affected part with cold water and ice as soon as possible.

 

VII. Evacuation Plan

 

·      Should conditions in the laboratory require evacuation due to an accident, hazardous chemical spill, fire or due to fire alarm sounding elsewhere in the building, students will immediately leave the building

·      Use the north stairway. Turn left upon exiting the building and proceed to the lawn across from the parking space on the west side of the Folsom Hall.

·     It is important that the class gather together at this location for the instructor to confirm that all students in the course have evacuated the building.

 

Laboratory  Etiquette

 

Lab can run smoother if everyone observes a few simple rules.  Individually these items take little effort, but when left to your instructor, it adds significantly to laboratory preparation time.  Carelessness also affects other courses using the facility so be considerate as you use the lab.

 

Returning prepared slides to their boxes. 

·        Take one slide at a time,

·        Return it to the same box

·        Place the slide in the box with the label on the left and facing the box front.

·        Put the slides in the box in slots directly opposite.  Don’t put the slide in at an angle.

 

Dispose of your trash in the proper receptacles.

·        Do not leave matches, tissues and pH paper in the trays.

 

Wipe down all bench space you have used during lab period.

·        If you make a mess at your bench, wipe it up with a sponge or paper towel.  

·        Wipe down benches you have used for solutions and equipment with a sponge and soapy water. 

 

Return dissecting tools, test tubes and other equipment to their proper place.  If you don’t know where it goes, ask.

 

Cleaning glassware (slides, tubes, beakers etc.)

 

·        Remove all tape and label.  Wax pencil marks come off readily with a moist paper towel and a bit of elbow grease.

·        Use soapy water to wash the glassware

·        Rinse with tap water and again with distilled water (In wash bottles).

·        Sort beakers and such by type (plastic or glass) and size when returning to their proper shelf or drawer.   If you don’t know where they go, ask.